Exploring the Synthesis, Characterization, and Anti-Cancer Efficacy of L-Lysine and TEMPO Coated Silver Nanoparticles

Abstract

This study focuses on the synthesis and characterization of silver nanoparticles (Ag NPs) coated with L-Lysine monohydrochloride and TEMPO (4-Amino-2, 2, 6, 6-tetramethylpiperidinyloxy, free radical) using a sol-gel route method with sodium borohydride and silver nitrate (Ag NO3) at various temperatures. Morphological analysis using scanning electron microscopy revealed nanoparticle sizes ranging from 0.5?m to 1?m, with aggregation attributed to improper grinding of the powder sample and particle agglomeration during chemical synthesis. The study observes a continuous increase in nanoparticle size due to the coating of L-Lysine and TEMPO. X-ray Diffraction (XRD) data, analyzed using the Debye Scherrer equation, confirmed the structure of the nanoparticles, supported by previously published data and JCPDS cards. Fourier-transform infrared spectroscopy (FTIR) revealed characteristic functional groups, with notable changes attributed to the coatings. Anti-cancer activity was evaluated using a cellular model (Hepatocellular carcinoma “HCC”), showing a decrease in cell viability with increasing nanoparticle concentration, particularly noteworthy under dark conditions. Further investigations will explore the potential of these nanoparticles under laser irradiation techniques. This research suggests the promising application of L-Lysine and TEMPO-coated silver nanoparticles in cancer therapy, particularly in targeted approaches.